15 Questions 8 Answers 0 Followers
Questions related from Mohammed Ahmed
Hello, I am investigating the role of microglia activation on GL261 cells. I would like to perform a functional experiment. I have optimized microglia cells isolation from mouse brains but I...
12 April 2021 8,032 0 View
Dear Researchers, I am going to run RNA Sequencing on mouse brain samples. I have collected the samples and then I put them in the RNALATER solution and stored them at -80. Following this...
07 October 2020 6,600 3 View
Dear All, I am looking for the best kit that could be used for RNA isolation ( High RIN) to perform RNA sequencing. any suggestions will be very helpful. Thank you very much.
14 July 2020 5,978 3 View
Dear Researchers, I am working on an animal study and I want to produce high quality of tissue imaging. The probelm, is that I have low quality of cryosectioning which can be noticed after...
18 December 2018 6,105 3 View
Dear Researchers, I am working with a PDCL cancer cells and I want to transfect GFP in the cells. I am using lipofectamine 2000 and I incubated it with the cells for 6 hour and seems to be good...
17 September 2018 6,122 3 View
I am trying to evaluate the in vitro activity of certain monoclonal antibodies against Glioblastoma solid tumor. I am wondering about the mechanistic theory? Would it be possible to test the...
12 April 2018 7,968 2 View
Dear Scientists, Currently we planned a new project which is involving few surgical techniques. Steriotaxic sugery in mice and introducing alzet pump to mice brains as well. I've seen different...
23 March 2017 2,609 7 View
Dear Researchers, I am in the process of writing a proposal and I am looking for an answer for this question in order to complete my research plan. "What is the morphological and the biochemical...
23 January 2017 9,035 3 View
Dear Researchers, From Pharmaceutical point of view: Could it be possible to have liposomal form of certain protein to be delivered to the brain or not? What is the practical issues related to...
25 November 2016 8,531 3 View
Dear Researchers, Could you provide me with a data that can answer this question ? "What is the exact dimensions of 6 months mouse's Hippo-campus" Regards, Mohammed
21 November 2016 1,512 1 View
I am working to determine the proper therapeutic dose for certain antibody so I need to know how we can evaluate the toxicity using a validated method ? Please, could anybody also provide me...
03 June 2015 6,497 4 View
I am working on splenocytes derived from healthy murine to investigate the effect of some compounds on interleukins and cytokines. I am using Con A as the mitogen to stimulate the lymphocytes...
12 April 2015 6,205 2 View
We are currently working on histone extraction from brain tissue, However, I need to know the concept of each step! from where could I get it ? or If could you provide me with it here? For...
24 March 2015 9,846 9 View
Dear All, I am going to use EvaGreen®/ Bio-Rad system to detect two genes using two pairs of primers. The first pair was successfully detected previously using EvaGreen® master mix. It was...
01 January 1970 8,665 4 View
Dear Researcher, I am investigating new therapeutic approaches to treat Glioblastoma. By testing my approach, I found that in one mouse model the data are promising. However, I am looking for...
01 January 1970 7,582 7 View
