Mohammed Ahmed

10 Questions 6 Answers 0 Followers

Questions related from Mohammed Ahmed

What could be the problem with my RNA extraction using RNeasy Mini KIt from Qiagen ?

Dear Researchers, I am going to run RNA Sequencing on mouse brain samples. I have collected the samples and then I put them in the RNALATER solution and stored them at -80. Following this...

07 October 2020 6,501 3 View

Which RNA isolation kit yields good quality RNA from mouse tumor to perforrm Illumina RNA seq analysis?

Dear All, I am looking for the best kit that could be used for RNA isolation ( High RIN) to perform RNA sequencing. any suggestions will be very helpful. Thank you very much.

14 July 2020 5,883 3 View

How to increase the brain tissue integrity during the harvesting?

Dear Researchers, I am working on an animal study and I want to produce high quality of tissue imaging. The probelm, is that I have low quality of cryosectioning which can be noticed after...

18 December 2018 5,949 3 View

Transfection Problem with a patient derived cell line?

Dear Researchers, I am working with a PDCL cancer cells and I want to transfect GFP in the cells. I am using lipofectamine 2000 and I incubated it with the cells for 6 hour and seems to be good...

17 September 2018 6,014 3 View

What is the best device for Steriotaxic Surgery in Mice?

Dear Scientists, Currently we planned a new project which is involving few surgical techniques. Steriotaxic sugery in mice and introducing alzet pump to mice brains as well. I've seen different...

23 March 2017 2,548 7 View

Could it be possible to have liposomal form of certain protein to be delivered to the brain?

Dear Researchers, From Pharmaceutical point of view: Could it be possible to have liposomal form of certain protein to be delivered to the brain or not? What is the practical issues related to...

25 November 2016 8,471 3 View

What is the exact dimensions of 6 months mouse's Hippo-campus ?

Dear Researchers, Could you provide me with a data that can answer this question ? "What is the exact dimensions of 6 months mouse's Hippo-campus" Regards, Mohammed

21 November 2016 1,460 1 View

What is the best way for assessment of protein levels of TGF beta1 in splenocytes after challenging with test compound and con A?

I am working on splenocytes derived from healthy murine to investigate the effect of some compounds on interleukins and cytokines. I am using Con A as the mitogen to stimulate the lymphocytes...

12 April 2015 6,153 2 View

How to choose the Tempreture in EVAGREEN Multiplexing digital droplet PCR ?

Dear All, I am going to use EvaGreen®/ Bio-Rad system to detect two genes using two pairs of primers. The first pair was successfully detected previously using EvaGreen® master mix. It was...

01 January 1970 8,605 4 View

Which of the following is considered a convincing scientific evidence in vivo?

Dear Researcher, I am investigating new therapeutic approaches to treat Glioblastoma. By testing my approach, I found that in one mouse model the data are promising. However, I am looking for...

01 January 1970 7,522 7 View