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Questions related from Muhammad Ali Haidar
I want to check if I can use GFP mice for IF quantification and look for proteins using secondaries that wouldn't excite GFP? (568 or 532)? Would GFP signal interfere with my staining and...
05 January 2022 5,172 2 View
Hello everyone, I want to do IHC on brain free-floating sections. Has anyone tried this before? If yes, what's the best protocol. I usually do IF using these sections, but I want to try IHC....
03 September 2021 8,823 4 View
Hello I am working on mice free-floating brain tissue (40um) and want to perform staining of specific membrane and cytoplasmic markers. The current protocol I am using is producing a lot of...
09 January 2021 487 6 View
Hello, I am trying to isolate the mitochondria from brain tissue to see the effects of a certain drug administration on mitochondrial complexes and respiration. How can I extract the mitochondria...
02 September 2020 4,615 3 View
Hello, I want to prepare brain tissue for western blotting. I have the hippocampus extracted and frozen in liquid nitrogen. I tried to crush it using a tissue pulverizer but I was afraid that...
31 July 2020 5,932 3 View
