How to dissolve cage-shaped silsesquioxane?

13 January 2021 1,190 3 View

Equipment for immunohistochemistry in free floating sections?

So I have been doing IHC on sections mounted on glass slides for a while. Lots of advantages to this, but one disadvantage is double labeling of proteins in single neurons. For this part of my...

09 December 2019 6,451 2 View

Can a city become more competitive if more demand is added to its main roadway?

Considering that transport is the main component of the logistics system, making domestic production more competitive is basically a strategic decision. How can increased demand for a city's...

20 May 2019 4,193 17 View

What are the consequences of urban dispersion in cities?

I'm reading Alain Bertaud's book - Order without Design: How Markets Shape Cities - and much is discussed about urban planning and, given the circumstances, what decisions should planners make?...

23 March 2019 4,118 9 View

How to remove stuck frit inside HPLC column?

I have stuck frit inside an HPLC column I want to remove, anyone knows how to remove it?

16 February 2019 5,514 6 View

Absorbance / Emission of qubit BR DNA assay?

Does anyone know the abs/em of qubit's broad range dsdna assay? I have a lot of leftover reagents, and no qubit, but I do have access to a plate reader.

23 April 2018 8,271 2 View

Building RadSeq library with gDNA isolated without RNase treatment. Anyone suspect an issue?

Hi, I'm making RADseq libraries using fish fin clips that have been stored in a chaotropic buffer (4M Guanadinium thiocyanate + detergent, EDTA, TRIS and B-ME). Ideally, I'd like to homogenize...

08 March 2018 3,765 2 View

How do I calculate the absorbance for BBCEAS-like systems?

15 September 2017 6,473 1 View

Do you know any protocol for the isolation of phages for staphylococcus aureus from nasal swap?

20 June 2017 9,832 1 View

No title

Hi, Some weeks ago I sent some paraffin blocks and sections (mouse tissues from a very important experiment) to another lab and just today I was informed that the person who received the package...

03 January 2017 6,247 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View