Dayan Lozano

5 Questions 4 Answers 0 Followers

Questions related from Dayan Lozano

Is possible to change LB broth for trypticase soy broth after transformation of the E. coli DH3? Having any consequence?

I wonder if having any consequence change the LB medium after bacterial transformation and to use any other enriched broth, for example, trypticase soy broth.

27 November 2020 3,673 3 View

What's the best human macrophage cell line for estimation of the diferential gene expression of S. aureus during a infection celular model?

I need to compare the gene expression of S. aureus during infection celular model.

31 July 2018 1,144 2 View

A method for collect nasal samples for Metagenomics analyzes diferent to UVTM?

I will take a nasal samples for to search Bacteriophages, but i whant to take a aliquot that for a future to realize metagenomics studies, which the better method for to take this samples. The...

09 November 2017 6,083 2 View

Do you know any protocol for the isolation of phages for staphylococcus aureus from nasal swap?

I tried to isolate phages from nasal swab using the Kropinsky´s 2009 protocol with LB + CaCl2 and using the strain RN4220 host. You know others protocols for this purpose.

21 June 2017 9,907 1 View

Someone meet a protocol to isolate bacteriophages to infecting S. aureus from nasal swab samples?

I want to isolation phages to infecting S. ausreus, from nasal sawb samples, but i don´t found a protocols. Thank´s

09 September 2016 7,208 2 View