Hi,
We are setting up a new lab and we are having a hard time trying to determine what MAC desktop specs we need for structural protein research, using Coot, python and Phenix mainly. Any suggestion is more than welcome thank you,
My cells are forming a rare clump during harvesting. I harvest at 80 to 90% confluence using 1X trypsin (0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks' Balanced Salt Solution with...
21 November 2023 5,229 1 View
Octakis(trimethylsiloxy)silsesquioxane is reported to be sparingly soluble (0.61 g/L 25°C Calc). I tried using organic solvent such as toluene, hexane, DCM and chloroform, however the powder...
13 January 2021 1,323 3 View
I wonder if having any consequence change the LB medium after bacterial transformation and to use any other enriched broth, for example, trypticase soy broth.
26 November 2020 3,695 3 View
Hello all, I have TEMPO radical and I want to manipulate it in a way the it will no longer be a stable radical, do you know if that can be done? Alternatively, do you know of a material to TEMPO...
14 June 2020 4,455 0 View
So I have been doing IHC on sections mounted on glass slides for a while. Lots of advantages to this, but one disadvantage is double labeling of proteins in single neurons. For this part of my...
09 December 2019 6,590 2 View
Considering that transport is the main component of the logistics system, making domestic production more competitive is basically a strategic decision. How can increased demand for a city's...
20 May 2019 4,469 17 View
It may seem that city planners and designers are most interested in understanding the process of transformation of cities and towns. However, commissioners, developers, consultants, interested...
26 March 2019 3,025 4 View
I'm reading Alain Bertaud's book - Order without Design: How Markets Shape Cities - and much is discussed about urban planning and, given the circumstances, what decisions should planners make?...
23 March 2019 4,337 9 View
I have stuck frit inside an HPLC column I want to remove, anyone knows how to remove it?
16 February 2019 5,876 6 View
Hello all, I'm using an home-built HPLC column and the pressure goes to the maximum even with very low flow (0.01 ml/min). Do you have any suggestions as to how to reduce pressure? Perhaps I...
03 September 2018 7,763 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...
04 August 2024 3,196 2 View
My collegue and I are having hard time to understand what is happening. Basically we have a tapping device that work with Arduino and a pneumatic pump. The pump allows the tapping of two...
04 August 2024 4,662 1 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,913 6 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
In molecular dynamics simulation, to get FEL analysis, I got an error. My Python version is 3.10.7. My input files are made with a lower version of Python. But the final command to generate the...
23 July 2024 5,646 2 View