How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to model rupture in geogrid in abaqus?

Hi researchers I wanna model tensile test on geogrid in abaqus, so I added displacement to one side of geogrid and the other side of geogrid is constrained completely, everything seems normal but...

19 February 2021 2,195 2 View

Any methods to train a neural network with random (stochastic) and non-measurable variable?

Dear scholars, I was wondering if there are any scientific methods to train a neural network or ANFIS when one of the input variables is stochastic (random) and more importantly non-measurable?...

17 February 2021 1,909 3 View

How to generate MATLAB code from ANFIS model?

Dear scholars, I was wondering how can I generate MATLAB code of an ANFIS (FIS) model? I need to integrate my ANFIS (FIS)/Fuzzy logic system into another MATLAB code to render optimizations. Do...

04 February 2021 6,098 4 View

In the tensile test for a nanocomposite sample, if the sample width decreases (thickness is constant), what does the stress-strain curve change?

Given that the stress is equal to the force divided by the area(width*thickness), what change does the reduction in sample width make to the stress-strain curve? Does stress change occur...

09 January 2021 5,124 5 View

How to convert Shamsi dates into Christian dates in SPSS?

Hi I have a database in which there are several dates indexed as strings and in Shamsi format. I need them as dates (not strings) and prefer them to be Christian. I have tried "Date and Time...

14 December 2020 2,798 3 View

Does anyone know a free large dataset from smart factory with data collected from IoT objects that I can use in academic research?

I am working on the integration of the Internet of Things into business processes. To evaluate my method, I need a large dataset with data collected from different kinds of objects. Thank you!

08 December 2020 7,338 4 View

Can anyone help or advise how and by what mechanism graphene oxide-activated persulfate can react with acrylamide and polyacrylamide in water solvent?

What are the products of the reactions mentioned above? And by what mechanism are they produced? Does graphene oxide itself participate in the reaction? Does not sulfate react with graphene...

18 November 2020 6,938 6 View

How to simulate locking range of an injection locked frequency doubler in cadence ?

Hi im trying to simulate an injection-locked frequency doubler (15 to 30 GHz) in cadence, I'm plotting DFT of transient signal to calculate injection locking range or to see injection pulling...

14 November 2020 896 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View