Hi,
I am new to primary cell culture and I'll be working with chicken-derived dendritic cells. I'll be using GM-CSF and IL-4 to induce their maturation in 6-well plates with RPMI-1640 media. I would like to know what's the recommended volume for media in 6-well plates? and also what's the recommended volume for GMCSF and IL4 on 6 well plates when doing cell culture?
If you are new to cell culture, check out this table, it's a great go-to guide (not just at the beginning).
If you are new to cell culture, check out this table, it's a great go-to guide (not just at the beginning).
Hi Keila,
In general, you will also have to consider on the number of days which you are going to incubate your cells in the incubator as a longer incubation period may eventually lead to your media dried up if it's too little. I would usually add 2-3mL in total when I am using 6-well plates.
All the best!
we usually used 1.5 ml per well in our cultures, but the medium was renewed after 2 days and at the end of the experiment (day 4 after seeding), when measuring the level of liquid present in the well, we usually got 1. 1-1.2 ml left
The number of PC 12 cells at the end of the experiment was usually around 1 million
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