Hi,
I'm working on a project where the main objective is to measure the maturation markers of chicken-derived dendritic cells. Naturally, I am looking at CD40, CD80/86 and MHCII markers via flow cytometry. However, I been having troubles finding my required chicken CD40 antibody for FACS because it has been discontinued in many companies and/or the available ones are not reactive with chickens. I have checked with companies like abcam, biorad, southern biotech, and many other antibody producing companies, but no luck. Does anyone know any other source where they might have this antibody available? Any suggestions are highly appreciated.
Thanks in advance
The only one we know of right now is mAb AV79 from US Biological. Our listings are here: https://www.linscottsdirectory.com/antibodies?q=chicken%20cd40
Just follow "more info" to suppliers' pages.
Hope that helps.
I called and they informed me it is discontinued there too. Thank you for your help though.
In case you are still struggling, you could try staining the RNA of CD40 on your FACS. Thermo has something called PrimeFlow RNA but other manufacturer may have similar products that I am not aware of.
I've worked with a customer facing a similar issue while staining IFNg and TNFa on guinea pig splenocytes. No antibodies. RNA staining worked like a charm.
Hope this helps.
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