Keila Acevedo Villanueva

4 Questions 3 Answers 0 Followers

Questions related from Keila Acevedo Villanueva

Can anyone give me a working protocol to extract DNA from Salmonella colonies using chloroform-isoamylalcohol (24:1)?

I need a working protocol to extract DNA from Salmonella colonies.

06 June 2018 821 4 View

Whats the recommended volume for 6-well plates in cell culture?

Hi, I am new to primary cell culture and I'll be working with chicken-derived dendritic cells. I'll be using GM-CSF and IL-4 to induce their maturation in 6-well plates with RPMI-1640 media. I...

02 February 2018 6,834 5 View

Does anyone know where can I buy chicken CD40 antibody for FACS?

Hi, I'm working on a project where the main objective is to measure the maturation markers of chicken-derived dendritic cells. Naturally, I am looking at CD40, CD80/86 and MHCII markers via flow...

02 February 2018 5,361 4 View

Can you make 50 ng/mL IL-4 from a stock of 10ng/mL IL-4?

Dear All, If a person has a stock solution of IL-4 at 10 ng/mL in a 50 mL conical tube and needs to add IL-4 at 50 ng/mL to 2 mL of media, can this stock be used for this purpose? I'm asking...

02 February 2018 6,234 1 View