Does anyone know where to order the dye Direct Yellow 96 (Solophenyl flavine) in Europe ?

I cannot find any supplier in Europe of this dye. Than you !

04 October 2020 9,305 2 View

How to perform contigs alignment to an homologous chloroplast reference?

I recovered an N amount of putative chloroplast contigs (fasta format). I need to align them to a reference genome and there is no reference genome of my species. Fortunately, there is a reference...

20 August 2020 1,162 3 View

488 laser emission crosstalk?

Image shows green fluorescence but no green fluorochrome was used in this specimen (HEK 293 cells), only Hoechst (350 nm absorption). Lasers used: 350 (upper right), 488 (upper left) and 568...

10 March 2020 7,321 3 View

Have you worked on a research project of Artificial intelligence?

At UMNG We are currently working on a Project of artificial intelligence from different prospective including ethical and legal consequences as well as artificial intelligence applied to education...

11 February 2019 1,821 3 View

Apathy and impulsivity scales for Parkison Disease?

16 March 2016 8,481 4 View

Have you ever thought why after reading 50 papers (including narrative reviews) about a research topic you don't even know all the variables affecting the process?

Why is information missing in the articles?

05 July 2013 6,483 4 View

Sites for exchange of skills or access to equipment.

Does anybody know if there is something similar to this but for UK based labs? https://www.scienceexchange.com/welcome

12 February 2013 9,383 2 View

Anyone know how I can lyse an amoeba without damaging the bacteria inside?

Viability assays

17 January 2013 5,147 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

No title

22 February 2021 9,932 11 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View