I'm performing a digestion with SmaI but when I concentrate the digested DNA with a Zymo Kit I obtain half of the inicial DNA. I'm working at 30°C like the insert says.
I am required to evaluate datasetsfrom other sources such as online databases and websites. However this is rather challenging to find. The evaluation of the datasets aims to inform the question...
25 June 2024 9,375 6 View
I had to run a gel electrophoresis at college with an undigested pUC18 (all the way left), digested pUC18 (lane 7) and a digested version (lane 8) in which i inserted a fluorescent gene (pET...
23 May 2024 7,103 2 View
I have been trying to check on literature regarding the shelf-life of a plant extract (Tulbaghia violacea) which may be used for the purposes of green synthesis, however, I have not found any...
10 April 2024 5,101 5 View
I can't find at what pH I have to adjust the CaCl2 solution. In my notes it's necessary 8.0 but i'm not sure
28 March 2024 4,650 1 View
Dear all, Hello, I am recently working on isolating neutrophils from murine bone marrow by using Histopaque 1099 and histopaque 1077, but the purity of the cells are always unsatisfactory. Here I...
01 February 2024 9,202 3 View
The gold nanoparticle was purchased from the company and the concentration was given as 6.0E+12 particles/mL. I was wondering if I can simply use Avagadro's number to convert it to nanomolar. The...
28 May 2023 5,737 3 View
Hello, do you have any suggestions for cleaning a quartz tube used for hBN growth? The precursor I am using is borazane (H3BNH3). My precursor was placed outside the furnace and, following my...
14 July 2021 7,033 2 View
I need a brief explanation of what should be the focus of the study today in the discipline Public Administration.
08 June 2021 4,235 22 View
La gamificación es una técnica de aprendizaje que traslada la mecánica de los juegos al ámbito educativo-profesional con el fin de conseguir mejores resultados, ya sea para absorber mejor algunos...
27 December 2020 3,243 2 View
I cannot find any supplier in Europe of this dye. Than you !
04 October 2020 9,498 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,572 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,056 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,541 11 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,891 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,647 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,228 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,556 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View