Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

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26 December 2020 1,951 3 View

What is the recommended tool for quality assessments of case reports?

I am performing a systematic review and the majority of published papers are case reports. In the same way we use the Newcastle-Ottawa scale (NOS) to evaluate the methodological design quality of...

13 September 2020 2,373 5 View

Is there any research on in-vitro meat about sporadic cancerous cells and their possible impact?

The high rate of proliferation required of stem cells could produce genetic instability which may result in sporadic cancerous cells. Is there any research on this?

10 September 2020 3,666 1 View

What is the proper solvent for crystallization of molebdenum diselenide in solution and room temperature other than DCM?

For crystallization we need a very good solvent to make a very clear solution, at least a good quantity of our solute is dissolved. However in our case we have to use an anhydrous solvent that...

17 June 2020 4,562 4 View

488 laser emission crosstalk?

Image shows green fluorescence but no green fluorochrome was used in this specimen (HEK 293 cells), only Hoechst (350 nm absorption). Lasers used: 350 (upper right), 488 (upper left) and 568...

10 March 2020 7,321 3 View

Unusual annealing temperature of primers in rt-PCR??

Hi everyone, It may not be a problem really but...I was very surprised because although in manuals and from my experience, I know that the Ta can differ up to 5 degrees or more from the Tm of the...

19 November 2019 8,770 3 View

Flow cytometry -advance pipeline & compensation?

Hii! So I designed for my next experiments a quite complex panel and I have a few questions about how better proceed for treating my Abs and building my compensation. So I have a Fixable...

14 November 2019 6,590 3 View

Which parameter should I match to design a reduce sized scale model of a hydarulic turbine?

Hi, I'm trying to design a reduced scale model of a turbine tested with glycerol. The prototype idea is to install a turbine in tailings flux to recover a percentaje of energy from it. The...

22 October 2019 9,239 3 View

How education and entrepreneurship is articulated in your country?

In Ecuador, entrepreneurship is in vogue. The country has a legal framework that normalizes the articulation of Education and Entrepreneurship. In addition, Ecuador has the highest rate of Total...

15 July 2019 7,807 6 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Database of European Football Players Salaries ?

Hello everyone, I am interested in writing about a dissertation about the wages of the football players of the 5 main leagues in Europe. I wanted to know if someone had an up to date data base...

17 February 2021 8,847 3 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View