Do you have any suggestions for me to modify my LS-DYNA model?

Hi, I use LS-DYNA to simulate the steel composite shear wall now. I use MAT_084/085 to simulate concrete, MAT_159 to simulate steel plates, and MAT_024 to simulate studs. The concrete section...

24 January 2021 3,863 1 View

Why there is a band around 25Kd of caspase3 in both undifferentiated and differentiated PC12 cells?

Dear all, When I detect the protein expression of caspase3 in PC12 cells, there is always a band around 25Kd. According to literature, the pro-caspase3 should be 35Kd and the cleaved caspase 3...

21 September 2020 8,577 2 View

How to claim back your publication?

My publications have been wrongly claimed by other ppl and I could not claim it back. Browsed through all the pages/tabs, but still could not find where to make the complain. Anyone knows how to...

19 August 2018 7,538 13 View

Does anyone have information about plane failure on rock slope?

07 April 2016 8,054 3 View

What is the NRTL model in chemical engineering?

I'm wondering what is the NRTL model and its usages. I wanna know about NRTL HOC, as well. Please let me know. Thanks.

27 June 2015 5,427 3 View

How to get the standard redox potential of CO2 + 6H+ + 6e- -CH3OH + H2O -0.38 V vs NHE at pH7?

08 February 2015 2,269 3 View

Is it possible to measurement Ca2+ concentration in neurons using a microplate reader?

I want to use fluo-3 to detect the intracellular Ca2+ and I used spectramax M5. Can I use serum-free medium as the buffer?

18 December 2014 4,361 3 View

How to set the parameters for valence band XPS checking?

Normally, we only check the survey XPS and high resoulution XPS of different elements. How to check the valence band XPS? can we check the XPS VB by seting the survey scan range from 20 eV to -5...

01 January 1970 9,086 7 View

How to respond to biased reviews

It has been almost 15 years since my first submission of a manuscript for journal publication. Some of my recent experience, grateful and furious, makes me decide to write such a post. After...

01 January 1970 2,213 6 View

How can Virtual Reality be applied for pediatric imaging and radiology?

Virtual reality is an emerging and essential technology in computer science. It is also useful for medical cares such as surgical simulation and understanding viruses. But how could the virtual...

01 January 1970 6,274 3 View

How to get the insoluble fraction of PK digestion for mitochondria?

I am trying to check for some proteins if they are located in the inner or outer mitochondria membrane after treating the cells with a neurotoxin. I am using PK to digest the mitochondria, but I...

27 January 2021 5,145 2 View

Where did Mitoprot software go (download version)?

Hi all! I've been using local copy of Mitoprot software for prediction of gene localization. https://ihg.gsf.de/ihg/mitoprot.html I've updated my laptop and now need to download Mitoprot again,...

18 January 2021 4,368 3 View

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling?

How can I selectively repress glucocorticoid receptor translocation to the mitochondria in primary cell lines without altering nuclear GR signaling? Any thoughts on applicable small molecules to...

06 January 2021 4,324 2 View

Is it correct to expect the same quantitative result between SDS-PAGE and BN-PAGE analysis performed on the same sample?

Please, can anyone please help me with this issue? By BN-PAGE and western blot on DDM-solubilized mitochondria, isolated from cultured cells, I found that in treated cells the amount of CV...

05 December 2020 7,696 2 View

How to prevent protein aggregation in gel filtration (size exclusion) column ?

I am currently trying to run solubilised mitochondria extracts from mammalian cells through a gel filtration column in order to separate proteins by size & visualise protein complexes. My...

29 November 2020 1,483 5 View

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

05 November 2020 8,841 3 View

How fast is mitochondrial degradation by mitophagy in yeast?

Hi everyone, I hope everyone can help me with this. First, I am measuring mitochondrial membrane potential by flow cytometry using TMRE and MTG dyes. I think we are not experiencing problems with...

17 September 2020 8,748 4 View

Any suggestions on gene candidates for normalizing qPCR on mitochondrial studies?

I am keen to know the most suitable reference gene(s) to normalise the qPCR for understanding the basic biology of mitochondrial OXPHOS complexes.

27 August 2020 7,935 3 View

How to deal with two peaks in S. cerevisiae mitochondrial mass and MMP using flow cytometry?

Hi colleagues, I am trying to measure mitochondrial membrane potential and mitochondrial mass in S. cerevisiae with TMRE and mitotrackerGreen as dyes using flow cytometry. However, I always see...

27 May 2020 7,708 3 View

In which solvent should I reconstitute mitoTEMPO?

Dear colleagues, I was wondering which solvent should I use to reconstitute mitoTEMPO, I've seen I could use water or DMSO but I'm not sure about the stability of the compounds in the respective...

18 May 2020 2,882 3 View