I'm doing primary tumor spheroids to a drug screening. We're having success to make the spheroids but I'm not having succes to dissociate the spheroids to evaluate the cell viability (using trypan blue to count).
I tryed to use trypsin 5 and 10 min (37ºC) and Accutase 10 min (R.T.) and the spheroids not dissociated completely. Could you help me?
p.s.: Spheroids were plated with 5000 cells, so they are ver small to dissociate mecanically.
Thank you!