Conventional protoplasting methods include in the medium osmoprotectants to keep formed protoplasts stable. In the methods I've come across, the osmoprotectant of choice seems to be lactose. Reason being, the sugar cannot be metabolized by the G+ thermophilic organism being transformed. However, the use of lactose comes at a price. That is, lactose can significantly inactivate, say Kanamycin, at high temperatures. At this point the researcher is force to look for an alternative antibiotic (not desired) or look for an alternative osmoprotectant that may not have this effect.
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