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Conventional protoplasting methods include in the medium osmoprotectants to keep formed protoplasts stable. In the methods I've come across, the osmoprotectant of choice seems to be lactose....
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To my dismay I have looked and looked trying to find the most reliable way to make a 1% Brilliant Blue G-250 solution as a stock to make other staining solution from. Among its physical properties...
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I have written many recommendation letters and currently find myself annoyed at the formality of the whole thing. YES, it is appropriate in most cases but in others I feel extreme formality takes...
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For many decades now many bacteriologists have used yeast extract to successfully culture bacteria. Nowadays, we see multiple examples where yeast extract appears to elicit certain regulation...
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For some time I have been under the suspicion that my bacterial cells are undergoing "autolysis". The cells are grown in minimal media with at least 1% carbohydrate supplement. It is worth...
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