Hi everyone,
I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:
http://www-naweb.iaea.org/nafa/aph/purine-cd/html/allantoin/index.html
It mentions that I use 15 mL tubes, but since I am boiling the samples should I use glass tubes with a cap to prevent escape? Or can I just use plastic tubes like the ones here:
https://www.sigmaaldrich.com/catalog/product/sigma/cls430791?lang=en®ion=US&gclid=CjwKCAiAouD_BRBIEiwALhJH6N8rIxMywgI3I9_JTYtwa9QXhEQWZxoqdOGVB5SgVmAnVj8vTVvCPhoCBzQQAvD_BwE
Thank you!
Kindly check http://www-naweb.iaea.org/nafa/aph/purine-cd/html/allantoin/html/05_5.htm
Also check https://journals.sagepub.com/doi/pdf/10.1177/1091581810362805
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