Hi
I'm doing FACs for spleens from C57Bl/6 mice and I find 2 populations : CD45.2 + hi and intermediate.
What is the difference between them ?
Thanks
Hi I'm trying to generate murine DCs from NSG HLA A2 mice ,,, using 20ng GM-CSF However, all what I get is MHCII neg, F4/80 hi, which show they are macrophages ,,, So I wonder can I generate...
02 March 2019 9,284 4 View
Hi every one I wonder if I can differentiate between M1 and M2 macrophages in mouse tumours by using the following markers only? CD11b, CD11c, Ly6C, Ly6G, F4/80, MHCII If not ,,,which Ab I...
05 June 2018 7,500 2 View
Hi I need a good panel for my FACS experiment which I 'll differentiate (mature DCs- which pulsed with peptide) and immature DCs ? Thanks
04 May 2018 7,563 3 View
Hi I give my mice 15mg/kg of a specific drug So how many nanomolar I give to my mice If the drug's formula weight is 439.52g/Mol ?? Thanks
31 December 2017 6,649 3 View
Hi What does the different shape of cells mean, invivo subcuatneous, cells are from single sorting spindle shape and round shape of pancreatic cancer cell line from KPC mouse ? thanks
04 May 2017 5,578 1 View
How IFNg can increase the expression of MHCI in vivo? what about in vitro? Thanks
03 April 2017 361 4 View
I need to test the proliferation ability of anti-Her2 CAR T cells in response to Her2 expressing cell lines using 3H incorporation assay ,,, First I need to stop proliferation of my cancer cells...
03 April 2017 5,860 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View