I have isolated Lin- CD117+CD150+Sca1+. But there are two types of cells here: CD48+ and CD48 -. What is the difference between them? Can I call CD48+ cells more differentiated HSC and the another one something like long-term HSC?
I briefly wanted to to reach out concerning your inquiry. I agree that your Lin‐cKit+Sca1+CD150+CD48- cells are the LT-HSCs whereas the Lin‐cKit+Sca1+CD150+CD48+ constitute a hematopoietic progenitor population which will differ in their self renewal capacity when transplanted in competitive bone marrow transplantation experiments. For details please see Figure 2 and supplementary data in:
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In addition, please bear in mind that these populations will differ between immunocompetent and deficient mouse strains, underlying their hematopoietic defects. For details please consider recent work from the Levesque lab.