Can anyone support me and discussion the agilent HPLC operation ?

I would like to exchange the information and skill about Agilent HPLC apparatus

06 July 2019 7,995 7 View

What the suitable protocol to analysis the solvent like methanol of acetic acid (organic acid)?

What the suitable protocol to analysis the solvent like methanol of acetic acid (organic acid)? Can I detect the concentration of residue methanol by HPLC?

06 July 2019 1,358 3 View

Cytochrome P450 evaluation?

what the best procedure to detect the activity of p450 of mice?

08 September 2018 2,024 0 View

What the best and easily method to prepare liposome nanoparticles?

what?

02 March 2018 9,567 0 View

What the modren stem cell application

The key properties of a stem cell were first defined by Ernest McCulloch and James Till in the early 1960s. They discovered the blood-forming stem cell, the hematopoietic stem cell (HSC), through...

01 January 1970 3,313 24 View

What the best solvent to remove the oil from herbs extract after extraction?

sometime we see an oil with the extrac Although polarity solvents are used.

01 January 1970 5,514 8 View

Why does cytochrome remove the effect or modulate drugs by oxidation of free radicals that also have effects?

Why does cytochrome remove the effect or modulate drugs by oxidation of free radicals that also have effects? glotathione then performs an antioxidant process by reducing drugs ACTION glotathione...

01 January 1970 8,684 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View