Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

Cross country comparison with matched pairs design?

Hi, I'm doing a cross-country comparison. My data for each country has a match pair design i.e. each company within a country is match with a control firm from that country. The data is...

07 January 2021 4,983 6 View

Stably transfected A549 cells are getting washed off during a plate based assay?

HI, I have transfected A549 cells with a plasmid of interest. The transfectants have been stably selected and clonally separated. I am doing a plate based fluorescence assay with the transfectants...

20 December 2020 1,222 4 View

What are the data analysis methods/techniques that can be used for matched pair data?

I have data in matched pair with one experimental group and one control group. I used conditional logistic regression for my analysis. Is there any other method which can be employed for data...

14 November 2020 9,034 2 View

Hello everyone,can we use content analysis method to measure environmental performance?

I am working on environmental and financial performance analysis of selected companies in India while reading several research papers i observed that environmental performance have been measured...

13 October 2020 527 11 View

Hello everyone, what are the sources of collecting secondary data regarding environmental performance and financial performance of co. in India?

While reading the annual reports of various companies I observed that some of the companies were following calendar year whereas others were following financial year.Since i have to do analysis of...

11 October 2020 2,584 7 View

No title

Hello Everyone, Any one having expertise in the associated field (MCDM - Manufacturing) mentioned in the flyer can contribute and recommend to others. Best Regards Dr. Pushpdant

05 October 2020 2,488 3 View

Is it appropriate to apply fixed effect model when the dependent variable is changing slowly over time for a given ID?

I am using a panel data set, where, my dependent variable is a score, moving slowly over time (24 years). Is it appropriate to apply a fixed effect model?. I have come across slides on "Panel...

27 July 2020 8,547 4 View

What are the good qualities of a teacher in current pandemic situation?

During the COVID-19 pandemic when all the teaching Institutions using online teaching methodologies. What are the qualities expected from the teacher?

12 July 2020 4,626 33 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View