I do phenol-chloroform RNA extraction. But after running the samples on gel electrophoresis, I see more DNA. What should I do to reduce genomic DNA during RNA extraction?
DNAse digestion and than another RNA extraction, with TRIsure or Phenol:Chloroform:isoamylalcohol 25:24:1
Abhijeet Singh Martina Colasante & Taijun Hana thank you all! I didn't use DNase during RNA extraction.
In my opinion, there is an enzyme that can remove the unwanted DNA in your RNA samples which is called DNase, you can use it .... Thank you,
The most common and effective method for removing trace to moderate amounts of DNA contamination from RNA samples is digestion with DNase I, a nonspecific endonuclease that selectively cleaves DNA (both single and double stranded) by hydrolyzing phosphodiester bonds. The DNase must be inactivated or removed from the reaction before subsequent PCR applications; otherwise, it may digest newly amplified DNA.
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