If you have not an appropriated method to quantify carboxylic acids an alternative methodology can be done by the process described above. In first step you use a specific reductive agent to be possible the quantitative transformation of each carboxylic acid to the corresponding aldehyde and after you add 2,4-dinitrophenylhydrazine to allow the detection of the derivative compound by the detector coupled to HPLC (using the stechiometry of the reaction you can know the amount of the carboxylic acids). However, your question is very general. Please, report your specific problem.

Hello i want biotin contain carboxylic acid group convert to amid by 2,4-dinitrophenylhydrazine but not result

Biotin has several groups that can be reactivity producing side compounds and probably this is the reason of your difficulties. I suggest you the foloowing reference to resolve your problem: Using of HPLC coupled with coulometric detector for the determination of biotin in pharmaceuticals Author(s): Zerzanova, A; Zizkovsky, V; Kucera, R; Klimes, J; Jesensky, I; Dohnal, J; Barron, D

Source: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS Volume: 45 Issue: 5 Pages: 730-735 DOI: 10.1016/j.jpba.2007.08.010 Published: 2007

Would any of these publications help you to solve your problem?

A.Kos ([email protected])

Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5'terminus

[PDF] from nih.gov

JN Kremsky, JL Wooters, JP Dougherty… - Nucleic acids …, 1987 - Oxford Univ Press

... than that of 16mer 14, the utility of this type of biotin labeling was ... CONCLUSION We have

described a two step DNA Immobilization procedure that Is applicable to any ... Beginning with a

readily prepared 5'-aldehyde or carboxylic acid containing oligonucleotide, a covalent bond ...

Cited by 107 - Related articles - All 10 versions

Covalent attachment and derivatization of poly (L-lysine) monolayers on gold surfaces as characterized by polarization-modulation FT-IR spectroscopy

[PDF] from uci.edu

BL Frey… - Analytical Chemistry, 1996 - ACS Publications

... have recently reported amide bond formation 19,20 and thioester formation 21 via activated MUA ...

of various reaction conditions by observing the percent conversion of the MUA carboxylic acids

to amide ... Previously, poly(l-lysine) has been reacted with NHSS−biotin in order to ...

Cited by 246 - Related articles - BL Direct - All 6 versions

Interaction between biotin lipids and streptavidin in monolayers: formation of oriented two-dimensional protein domains induced by surface recognition

R Blankenburg, P Meller, H Ringsdorf… - Biochemistry, 1989 - ACS Publications

... ture by the addition of a carboxylic acid group is also consistent with the observation of a looser ...

cific adsorption (Figure 3, dotted line) whereas specific binding of active streptavidin to biotin

lipid 7 ... pl - 1 l), streptavidin is not charged at neutral pH (pl - 7) and contains no sugar ...

Cited by 215 - Related articles - All 3 versions

A brief survey of methods for preparing protein conjugates with dyes, haptens and crosslinking reagents

M Brinkley - Bioconjugate chemistry, 1992 - ACS Publications

... a carboxylic acid or that can be chemically modified to contain a carboxylic acid can be ... Newly

developed NHS esters are available with sulfonate groups that have improved water solubility

(23). A shortlist of reactive NHS ester derivatives of fluorescent probes, biotin, and other ...

Cited by 460 - Related articles - All 6 versions

Versatile synthesis strategy for carboxylic acid-functionalized upconverting nanophosphors as biological labels

[PDF] from sxu.edu.cn

Z Chen, H Chen, H Hu, M Yu, F Li… - Journal of the …, 2008 - ACS Publications

... S5 of the Supporting Information) also demonstrate that both samples have similar phases ... Typically,

the terminal carboxylic acid groups allow the immobilization of amine-containing proteins and

DNA ... interaction (high affinity, K d = 10 - 15 ) between streptavidin and biotin, 23 in ...

Cited by 214 - Related articles - All 11 versions

Patterning ligands on reactive SAMs by microcontact printing

[PDF] from harvard.edu

J Lahiri, E Ostuni… - Langmuir, 1999 - ACS Publications

... The procedures for coupling were similar to those used for biotin. ... ligands on mixed SAMs

containing low mole fractions (χ(2) ≤ 0.10) of activated carboxylic acid groups. ... 18 The SAMs

contain patterned regions that present ligands at defined densities in environments that resist ...

Cited by 230 - Related articles - BL Direct - All 6 versions

Metal ion complexes with biotin and biotin derivatives. Participation of sulfur in the orientation of divalent cations

H Sigel, DB McCormick, R Griesser, B Prijs… - Biochemistry, 1969 - ACS Publications

... In the last- mentioned complex, Cu(1I) coordinated at the carboxylic acid group could only form

a ... some hydrogen occurs, so that nearly the intact dethiobiotin molecule is converted into biotin ...

Fe, are es- sential (Eisenberg, 1963); also it is known that biotin-contain- ing enzymes ...

Cited by 33 - Related articles - All 4 versions

Determination of the bioavailability of biotin conjugated onto shell cross-linked (SCK) nanoparticles

K Qi, Q Ma, EE Remsen, CG Clark Jr… - Journal of the …, 2004 - ACS Publications

... Furthermore, confirmation of the persistence of the biotin chain end unit was made through ... of

amine functional groups of the diamino cross-linker to the carboxylic acid groups from the ... D 3 .

Under the assumption that all particles in the volume-average distribution have the same ...

Cited by 122 - Related articles - BL Direct - All 8 versions

Chemical strategies for generating protein biochips

[PDF] from vanderbilt.edu

P Jonkheijm, D Weinrich, H Schröder… - Angewandte …, 2008 - Wiley Online Library

... By introducing a spacer between the protein and the reactive group on the surface, these ... or flexible,

hydrophilic or hydrophobic, charged or neutral.42, 111 It can even contain programmable

information, as we will discuss in the context of nucleic acid linkers (see ...

Cited by 116 - Related articles - All 12 versions

Characterisation of nitrilase and nitrile hydratase biocatalytic systems

[PDF] from csir.co.za

D Brady, A Beeton, J Zeevaart, C Kgaje… - Applied microbiology …, 2004 - Springer

... Kamal and Khanna (2001) have recently published a similar preparation for racemic β-hydroxy

compounds ... The vitamin solution was composed of (in g/l): biotin 0.05 (in 10 ml 0.1 mol/l NaOH,

adjust to pH 6.5 ... Table 2 Degree of complete hydrolysis of nitriles to the carboxylic acid. ...

Cited by 66 - Related articles - BL Direct - All 12 versions

Dibal-h