What should be the pulse length of electroporation for transfecting mammalian cells?

10 July 2017 1 2K Report

I am currently optimizing electroporation conditions for mouse embryonic stem cells. What should be ideal range of pulse duration?

Domenico Sanfelice

Attached there are some parameters you can set for electroporation: as you will see it depends on the type of cells and cuvette and temperature. You have to find the ones that best fit your system. Good Luck

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Science method

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