Bhakti Jayant Vyas

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Questions related from Bhakti Jayant Vyas

What should be the pulse length of electroporation for transfecting mammalian cells?

I am currently optimizing electroporation conditions for mouse embryonic stem cells. What should be ideal range of pulse duration?

10 July 2017 1,841 1 View

Has anyone used Biorad electroporation system to electroporate mouse ES cells E14Tg2a?

I wish to know electroporation conditions especially for transfecting Cas9 RNA and protein

19 December 2016 668 2 View

What is the appropriate raw CT value of GAPDH in qPCR?

I am getting CT value of 18-21 in 1 qPCR run and 17-18 in another. what is the desirable range?

05 September 2016 5,069 1 View

What should be the ideal ratio of primer to template DNA in PCR?

I have tried doing a PCR with kapa as well as Phusion HF polymerase. I am getting amplification with kapa, but Phusion is just not working. Both the master mixes use same: 1.5mM MgCl2 and 0.2mM...

29 May 2016 2,050 3 View

How should I genotype mouse embryos after in situ hybridisation?

We have tried doing genotyping PCR for some mouse embryos after complete in situ hybridization assay. But we did not get any band. I suspect something went wrong at lysis step because the positive...

14 March 2016 6,647 5 View