My His-tagged purified protein contains 10mM trisCl, 300mM NaCl and 200mM imidazole at which the protein was eluted at pH 8.0. What should be ideal dialysis buffer composition to remove imidazole?
I am using 10mM tris and 300mM NaCl at pH 8.0.
Does NaCl affect protein function? Do I need to remove it also?