How important Sustainable and Renewable Energy studies and dynamics in Europe ?

Dear sustainable and renewable energy researchers, I aim to review Research areas regarding Sustainable and Renewable Energy and I am currently establishing all the controls. Can you help me to...

11 January 2021 1,099 3 View

Is it possible to re-activate silenced transgene at T1 rice plants to get T2 seeds which is expressing the transgene gene again ?

I have three T0 plants (sblings) which were regenerated from same calli and expressing transgene simillar to actin level however when I analyze over 15 T1 plants I was not able to get any...

13 October 2020 4,077 3 View

Where can I collect high-resolution records, like stalagmite records and tree-ring records?

Dear scholars, I focus on the relationship between lake variation and paleoclimate change in the southern Tibet Plateau. And I want to collect some high-resolution records, like stalagmite...

24 October 2019 5,239 1 View

Is there any genomics company providing CAPP-Seq NGS analysis service for a research lab in Turkey?

Hi everyone, I plan to perform CAncer Personalized Profiling by deep Sequencing (CAPP-Seq) for a cancer research project. Is there any available genome analysis company that uses Illumina Hiseq...

09 February 2019 5,246 7 View

How can I obtain time zero-point when laser beam and electron beam inject into one sample ?

06 June 2018 7,824 1 View

Is there anyone who measures the pH of the medium during Saccharomyces cerevisiae growth in caffeine conditions?

Hello everyone, I am trying to answer some questions in my mind and I have to know what is the pH difference between start and stop point of growth of S. cerevisiae with caffeine. Thank you all.

17 October 2017 9,722 2 View

Is it possible to combined multi-temporal data and then classify it ? If it is , what are the steps of this process?

I have 5 Landsat8 images from 5 different time periods, I'm trying to use a different method other than post classification method. Basically I want to first combine the data and then classify....

27 September 2017 6,657 4 View

How photocatalytic oxidizing property of SnO2 changes, if its disolved in acid or base?

02 July 2017 7,652 3 View

How to dope nanoparticles?

14 October 2016 4,684 4 View

Greetings, what is the main factors to choose proper surfactant or capping agent to synthesis nanomaterial?

nanomaterial or nanophosphor

06 October 2016 10,004 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View