Is it possible to re-activate silenced transgene at T1 rice plants to get T2 seeds which is expressing the transgene gene again ?

I have three T0 plants (sblings) which were regenerated from same calli and expressing transgene simillar to actin level however when I analyze over 15 T1 plants I was not able to get any...

13 October 2020 4,077 3 View

Where can I collect high-resolution records, like stalagmite records and tree-ring records?

Dear scholars, I focus on the relationship between lake variation and paleoclimate change in the southern Tibet Plateau. And I want to collect some high-resolution records, like stalagmite...

24 October 2019 5,239 1 View

Can a meta-regression be computed on aggregate data (from multiple papers), where the assumed covariate was not reported in all papers?

It seems you can utilize a meta-regression from binary data, where differences in events vs. non-events stemming from treatment vs. control groups can be computed to an odds ratio (Thompson, Sharp...

14 September 2019 5,546 2 View

Is there any genomics company providing CAPP-Seq NGS analysis service for a research lab in Turkey?

Hi everyone, I plan to perform CAncer Personalized Profiling by deep Sequencing (CAPP-Seq) for a cancer research project. Is there any available genome analysis company that uses Illumina Hiseq...

09 February 2019 5,246 7 View

How can I obtain time zero-point when laser beam and electron beam inject into one sample ?

06 June 2018 7,824 1 View

What should be ratio of each of plp1,plp2 and plp_vsvg packaging plasmids in transfection mix?

Hi Currently i am using ratio of 1 ug pCMV-VSV-G to 9 ug pCMV-dR8.2 dvpr using pei to transfect HEK cells for lentivirus packaging . But i want to change it with plp1-plp2 -plp-vsvg...

28 January 2018 3,338 3 View

Is there anyone who measures the pH of the medium during Saccharomyces cerevisiae growth in caffeine conditions?

Hello everyone, I am trying to answer some questions in my mind and I have to know what is the pH difference between start and stop point of growth of S. cerevisiae with caffeine. Thank you all.

17 October 2017 9,722 2 View

Is it possible to combined multi-temporal data and then classify it ? If it is , what are the steps of this process?

I have 5 Landsat8 images from 5 different time periods, I'm trying to use a different method other than post classification method. Basically I want to first combine the data and then classify....

27 September 2017 6,657 4 View

How photocatalytic oxidizing property of SnO2 changes, if its disolved in acid or base?

02 July 2017 7,652 3 View

Is there any way for matching peaks in a database and find the compound (especially inorganics) in FTIR ?

FTIR peaks are in cm-1 ...

18 January 2016 5,895 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View