What should be ratio of each of plp1,plp2 and plp_vsvg packaging plasmids in transfection mix?

Hi Currently i am using ratio of 1 ug pCMV-VSV-G to 9 ug pCMV-dR8.2 dvpr using pei to transfect HEK cells for lentivirus packaging . But i want to change it with plp1-plp2 -plp-vsvg...

29 January 2018 3,518 3 View