Is it possible to re-activate silenced transgene at T1 rice plants to get T2 seeds which is expressing the transgene gene again ?

I have three T0 plants (sblings) which were regenerated from same calli and expressing transgene simillar to actin level however when I analyze over 15 T1 plants I was not able to get any...

13 October 2020 4,077 3 View

Where can I collect high-resolution records, like stalagmite records and tree-ring records?

Dear scholars, I focus on the relationship between lake variation and paleoclimate change in the southern Tibet Plateau. And I want to collect some high-resolution records, like stalagmite...

24 October 2019 5,239 1 View

Is there any genomics company providing CAPP-Seq NGS analysis service for a research lab in Turkey?

Hi everyone, I plan to perform CAncer Personalized Profiling by deep Sequencing (CAPP-Seq) for a cancer research project. Is there any available genome analysis company that uses Illumina Hiseq...

09 February 2019 5,246 7 View

How can I obtain time zero-point when laser beam and electron beam inject into one sample ?

06 June 2018 7,824 1 View

What should be ratio of each of plp1,plp2 and plp_vsvg packaging plasmids in transfection mix?

Hi Currently i am using ratio of 1 ug pCMV-VSV-G to 9 ug pCMV-dR8.2 dvpr using pei to transfect HEK cells for lentivirus packaging . But i want to change it with plp1-plp2 -plp-vsvg...

28 January 2018 3,338 3 View

Is there anyone who measures the pH of the medium during Saccharomyces cerevisiae growth in caffeine conditions?

Hello everyone, I am trying to answer some questions in my mind and I have to know what is the pH difference between start and stop point of growth of S. cerevisiae with caffeine. Thank you all.

17 October 2017 9,722 2 View

Is it possible to combined multi-temporal data and then classify it ? If it is , what are the steps of this process?

I have 5 Landsat8 images from 5 different time periods, I'm trying to use a different method other than post classification method. Basically I want to first combine the data and then classify....

27 September 2017 6,657 4 View

How photocatalytic oxidizing property of SnO2 changes, if its disolved in acid or base?

02 July 2017 7,652 3 View

Is there any way for matching peaks in a database and find the compound (especially inorganics) in FTIR ?

FTIR peaks are in cm-1 ...

18 January 2016 5,895 2 View

How to determine pad size, thickness and pad spacing for a TLM pattern for ohmic contact characterization to GaAs?

12 January 2016 304 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View