3 Questions 16 Answers 0 Followers
Questions related from Ahmad Jawad Sabir
I am trying to purify a Flag-tagged recombinant protein expressed in mammalian cells. I had packed a couple of columns for this purpose using sepharose resin and it worked fine before. Now I have...
28 August 2017 5,263 5 View
I have been doing affinity purification for a few months now, everything was working fine before but I am getting no protein with the newly packed columns. Antibody coupling efficiency is above...
08 August 2017 3,691 3 View
Has anyone experience of using expired sepharose resin. I have got resin expired 10 years ago but was stored properly at 4 C. Any suggestions or recommendations
23 June 2017 824 5 View
