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Questions related from Ahmad Jawad Sabir
I am trying to purify a Flag-tagged recombinant protein expressed in mammalian cells. I had packed a couple of columns for this purpose using sepharose resin and it worked fine before. Now I have...
28 August 2017 5,310 5 View
I have been doing affinity purification for a few months now, everything was working fine before but I am getting no protein with the newly packed columns. Antibody coupling efficiency is above...
08 August 2017 3,737 3 View
Has anyone experience of using expired sepharose resin. I have got resin expired 10 years ago but was stored properly at 4 C. Any suggestions or recommendations
23 June 2017 882 5 View
