I running SDS-PAGE and I am facing a problem i.e. if I am setting voltage 100V then current is going to much higher like 400mA. If I am going through current and setting it 35 mA then voltage is automatically going 7V. 

Gel is taking too much time to run, approx 9 hours. 

Can anyone suggest me what should be the actual value of it, because at 400mA nothing is visual in Gel.

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