Any advice on the Xylosidase of K. marxianus?

07 August 2015 5,201 8 View

How can I design a primer for Xylitol Dehydrogenase and Xylose Reductase genes of Kluyveromyces marxianus?

31 December 2014 8,800 2 View

What Should a proper ratio between Voltage and Current while running SDS-PAGE ?

I running SDS-PAGE and I am facing a problem i.e. if I am setting voltage 100V then current is going to much higher like 400mA. If I am going through current and setting it 35 mA then voltage is...

10 November 2014 2,639 4 View

What parameters should be in the primer design when designing primer for xyloser reductase and xylitol dehydrogenase gene?

08 September 2014 4,722 14 View

What is average value of Xylitol Dehydrogenase and Xylose Reductase activity assay (U/L)?

I am performing enzyme assay of Xylitol Dehydrogenase and Xylose reductase but I don't have any reference value for that. So tell me what satisfactory value of activity form ADH and XR can be...

07 August 2014 3,513 5 View

How do you design a vector for yeast transformation?

I am beginner in vector designing. My aim is to transform yeast for xdh and xi genes. so please provide me some references and protocols.

06 July 2014 9,824 1 View

How can I decide which restriction enzyme in better for restriction digestion?

Many restriction enzymes having same restriction sites so this is the problem that which is better and from where to search that enzymes.

05 June 2014 4,972 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View