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Questions related from NILESH KUMAR SHARMA
Dear All, I am trying to find amino acid sequence of beta xylosidase of K. marxianus, but it is not present in NCBI protein database. However, sequence of A. niger's xylosidase is available. So...
08 August 2015 5,267 8 View
Dear Friends, I am trying to design primer design a primer for Xylitol Dehydrogenase and Xylose Reductase gene of Kluyveromyces marxianus but when I am searching gene sequence in NCBI, it is...
01 January 2015 8,872 2 View
I running SDS-PAGE and I am facing a problem i.e. if I am setting voltage 100V then current is going to much higher like 400mA. If I am going through current and setting it 35 mA then voltage is...
11 November 2014 2,721 4 View
I am designing primer for xylose reductase and xylitol dehydrogenase gene. Can anyone suggest me what parameters should be their in primer designing. If anybody having protocol please provide me.
09 September 2014 4,786 14 View
I am performing enzyme assay of Xylitol Dehydrogenase and Xylose reductase but I don't have any reference value for that. So tell me what satisfactory value of activity form ADH and XR can be...
08 August 2014 3,623 5 View
I am beginner in vector designing. My aim is to transform yeast for xdh and xi genes. so please provide me some references and protocols.
07 July 2014 9,884 1 View
qPCR, 2D gel electrophoresis, Gene Cloning.
06 June 2014 1,446 2 View
Many restriction enzymes having same restriction sites so this is the problem that which is better and from where to search that enzymes.
06 June 2014 5,013 4 View
