What are your approaches and practices regarding games in the EFL classroom?

Dear fellow EFL teachers, we, Laura Schmetzer and Lennart Holzhuetter, are currently writing our master's thesis on the topic of games in the EFL classroom and would really appreciate it, if you...

28 July 2020 3,077 2 View

How can I export data (csv) from scopus effecient to an excel table?

Hey community i have a question for you. Im doing a literature review and i want to put the relevant literature in an excel table to check it later. Whenever i am exporting something in CSV (using...

13 January 2020 5,916 3 View

How does Burying beetle Nicrophorus vespilloides burying and copulating act look like?

I have read several papers about Nicrophorus vespilloides burying and copulation and I still have some questions. -Do only males bury the carrion? -Does male figth with intruders or also female?...

11 February 2018 8,481 9 View

Can you identify those bird feathers?

19 April 2017 2,715 3 View

Do I need permission to collect invertebrates from Gran Canaria?

I am going to Gran Canaria and planning to collect some invertebrates. Do I need permission for that and what should I do for getting the permission? I will collect for natural history museum of...

14 January 2017 5,777 15 View

Would you please identify those freshwater aquarium species?

20 October 2016 5,370 7 View

Can you identify those fish?

Please help me identify those fish. My oppinion 1-3 Taurulus bubalis 4-5 Cyclopterus lumpus

16 June 2016 725 6 View

What species of Mollusca are these specimens?

That species of Mollusca was found from lake Viljandi (https://www.google.ee/maps/@58.3483153,25.6106634,14z?hl=en). This summer. The measurements of shell are shell height 4,5mm shell width...

09 December 2014 2,540 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View