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Questions related from Lennart Zabeau
Dear all, For culturing CTLL-2 cells, ATCC recommends to use CORNING T-STIM with Con A as a source for IL-2. However, delivery (for all distributors) would take almost two months and we...
05 January 2022 2,984 3 View
Dear all, I am looking for a way to up-regulate CD25 expression in CTL cells, but without activating them. Ideally we stimulate cells for the up-regulation of CD25 on day 1 and on day 2 CD25 is...
21 April 2021 4,753 0 View
Dear all, I am looking for an Ab for staining the intracellular part of CD8 alpha in FACS. Possible ? Any suggestions ? Thx a lot, Lennart
26 November 2020 1,511 0 View
Dear all, I am trying make stable clones of a HEK293 using an antibiotic selection strategy. However, the parental cell line is already resistant to the five commonly used antibiotics (G418,...
15 August 2020 7,736 2 View
Dear all, Anyone knows what the principle behind KCM transformation of bacteria is ? Thx, Lennart
07 February 2019 6,693 2 View
Dear all, I have extensively searched the literature to gain more insights on how IL-12 binds to its receptor (binding-sites, residues involved, affinities, ...) but to my big surprise apparently...
01 January 1970 6,264 2 View
