What selection markers to use for the generation of stable HEK293 cell line ?

Dear all, I am trying make stable clones of a HEK293 using an antibiotic selection strategy. However, the parental cell line is already resistant to the five commonly used antibiotics (G418,...

14 August 2020 7,542 2 View

What are your approaches and practices regarding games in the EFL classroom?

Dear fellow EFL teachers, we, Laura Schmetzer and Lennart Holzhuetter, are currently writing our master's thesis on the topic of games in the EFL classroom and would really appreciate it, if you...

28 July 2020 3,077 2 View

How can I export data (csv) from scopus effecient to an excel table?

Hey community i have a question for you. Im doing a literature review and i want to put the relevant literature in an excel table to check it later. Whenever i am exporting something in CSV (using...

13 January 2020 5,916 3 View

How does Burying beetle Nicrophorus vespilloides burying and copulating act look like?

I have read several papers about Nicrophorus vespilloides burying and copulation and I still have some questions. -Do only males bury the carrion? -Does male figth with intruders or also female?...

11 February 2018 8,481 9 View

Can you identify those bird feathers?

19 April 2017 2,715 3 View

Do I need permission to collect invertebrates from Gran Canaria?

I am going to Gran Canaria and planning to collect some invertebrates. Do I need permission for that and what should I do for getting the permission? I will collect for natural history museum of...

14 January 2017 5,777 15 View

Would you please identify those freshwater aquarium species?

20 October 2016 5,370 7 View

Can you identify those fish?

Please help me identify those fish. My oppinion 1-3 Taurulus bubalis 4-5 Cyclopterus lumpus

16 June 2016 725 6 View

What species of Mollusca are these specimens?

That species of Mollusca was found from lake Viljandi (https://www.google.ee/maps/@58.3483153,25.6106634,14z?hl=en). This summer. The measurements of shell are shell height 4,5mm shell width...

09 December 2014 2,540 12 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View

Does anyone know papers that use large databases of abundance of different groups of species?

03 October 2020 2,506 4 View

Can anyone recommend a hydrophone for recording amphibian calls underwater?

I have a relatively small budget but I would like to purchase a hydrophone that would enable me to call and describe the vocalisations of frogs underwater. The animals I hope to record are in...

06 January 2020 4,280 3 View

Atypical colouration in caecilian amphibians?

I am curious to know about research publications discussing/reporting information on colour polymorphism in legless amphibians. Thanks in advance for your generous help. Venu

09 October 2019 444 0 View

Any suggestions for sample (tadpole liver) homogenization method before Ranavirus DNA extraction?

Briefly, I need a cost and time effective method - which completely exludes the possibility of contamination between samples - in order to get ranaviral DNA from the liver of EtOH stored...

17 September 2019 8,404 6 View

Any suggestions for sample (tadpole liver) homogenization method before Ranavirus DNA extraction?

Briefly, I need a cost and time effective method - which completely exludes the possibility of contamination between samples - in order to get ranaviral DNA from the liver of EtOH stored...

08 September 2019 5,526 7 View

How do I interpret a triallelic pattern in a diploid organism's microsatellite?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...

26 June 2019 9,059 2 View

How do I interpret a triallelic pattern in a diploid organism's microsatellite?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...

05 June 2019 1,890 2 View

How to quantify threats to the survival of species at Landscape Level?

I am interested to quantify threats to the survival of species at landscape level. But, I am unable to figure out the exact methods. On literature most of them just list the threats. I humbly...

04 May 2019 5,443 2 View

Hey , Any one help me? please point out the software important to analyse phylogenetic relation ship of amphibians?

I am studying Ethiopian amphibians now and I need the software to analyse the phylogenetic relation ship of amphibians.

04 May 2019 778 8 View