Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

Do you know any journal calling for papers related to education during covid-19 and post covid 19?

I have recently completed a research based article related to Managerial Challenges and Opportunities posed by COVID-19 and would like to publish this in an international journal within a month...

01 January 2021 5,614 3 View

How can I convert my *.m file to Simulink?

When the main m file (script) is executed around 5 files are loaded and values are assigned to variables, four user defined functions are also called and output is plotted in the graph inside the...

23 November 2020 4,288 3 View

What is the most sensitive Non-invasive prenatal testing for fetal sex determination?

Is 8th week the earliest week where we can determine the fetal sex ?

05 October 2020 9,576 9 View

For in silico analysis of a variant, howmany bioinformatic tools are required to give a sign in the favour of its pathogenecity?

Dear fellows, I am wondering about, how many bioinformatic tools are required to declare a variant as pathogenic and deleterious?

02 October 2020 6,580 3 View

Which model either moment hinge rotation or fiber hinge model can dissipates more energy ?

While doing energy based seismic design with non- linear time history analysis in perform 3D, our main target is to find the maximum amount of energy dissipation from structural components?...

23 August 2020 4,764 2 View

Will there be any conflict of interest or other issues if you publish in a journal in which you are an editor?

Is publishing in a journal in which you are an editor (associate or chief editor) considered a good practice? Are such practices regarded as okay in academic circle?

03 August 2020 2,714 7 View

Can you suggest the compound that can inhibit CCR6 in the rat for in-vivo study?

CCR6 inhibitor for rat in-vivo experiment.

03 May 2020 5,655 1 View

Applying Plant extracts on cell line?

Has anybody tried applying plant extracts on MCF 7 cell lines after filtering them? will it (filtering) effect the antioxidant activity present in plants?

12 March 2020 5,258 3 View

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02 June 2019 8,134 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View