Sher Bahadur Poudel

5 Questions 11 Answers 0 Followers

Questions related from Sher Bahadur Poudel

What proteins should I check to know impaired bone marrow microenvironments?

Hello everyone, I have been studying the bone marrow microenvironment for the maintenance of mesenchymal stem cells(MSCs). I am not so much clear that which proteins should I check to know the...

06 September 2018 4,848 3 View

What is the best method for isolating protein and mRNA from bone marrow?

Can anyone provide me a protocol for isolating mRNA and protein from the bone marrow of mice, rat and humans? I don't want to use any isolating kit.

20 September 2014 4,083 2 View

Can anyone assist with a question about Mouse bone marrow stromal cell sub culture?

I am isolating the bone marrow cells from mice and culture those cells. Every time when I try to subculture the cells, the cells do not detach. I tried using 0.05% Trypsin in 1X EDTA and also...

29 August 2014 553 5 View

What is the best method to analyze mouse bone marrow stromal cell culture?

I am having problem of mouse bone marrow stromal cells primary culture. First 2-3 days I see cells very healthy but then slowly hazzy and dying. I use alpha MEM media with 10% FBS and 1x glutamine...

13 August 2014 7,472 7 View

Can anyone suggest the literature explaining the senescence of bone marrow mesenchymal stem cells of diabetic mice model?

Hello everyone, There are lots of literature those explain about the senescence of mesenchymal stem cells by hyperglycemic conditions in in-vitro conditions. Also we can find the papers explaining...

01 January 1970 5,843 5 View