9 Questions 19 Answers 0 Followers
Questions related from Sher Bahadur Poudel
Hello everyone, I have been studying the bone marrow microenvironment for the maintenance of mesenchymal stem cells(MSCs). I am not so much clear that which proteins should I check to know the...
06 September 2018 4,870 3 View
I am trying to isolate dental pulp stem cells from mice teeth. I am getting heterogeneous population of cells in the culture. I want to isolate the DPSCs by cell sorting method. I went...
14 June 2016 8,266 5 View
Please I would like to know that, Is it good idea to culture Dental pulp stem cells(DPSCs) in the Alpha MEM media without L-ascorbic acid? I isolated DPSCs from mice molar teeth. Ascorbic acid is...
20 April 2016 8,050 6 View
Hello everyone, Do you know about the in-vivo model for dental pulp stem cell aging? I would like to know if there is any documented materials about the inducing factors for dental pulp stem cells...
29 December 2015 3,169 7 View
Does anyone know if FGF-7 has autocrine function?
25 March 2015 8,688 2 View
Can anyone provide me a protocol for isolating mRNA and protein from the bone marrow of mice, rat and humans? I don't want to use any isolating kit.
20 September 2014 4,107 2 View
I am isolating the bone marrow cells from mice and culture those cells. Every time when I try to subculture the cells, the cells do not detach. I tried using 0.05% Trypsin in 1X EDTA and also...
29 August 2014 579 5 View
I am having problem of mouse bone marrow stromal cells primary culture. First 2-3 days I see cells very healthy but then slowly hazzy and dying. I use alpha MEM media with 10% FBS and 1x glutamine...
13 August 2014 7,508 7 View
Hello everyone, There are lots of literature those explain about the senescence of mesenchymal stem cells by hyperglycemic conditions in in-vitro conditions. Also we can find the papers explaining...
01 January 1970 5,865 5 View
