I'm recording capsaicin evoked mEPSCs in dorsal horn slices and I'm curious which pain terminals these originate from. I gather from the literature that TRPV1 receptors are mostly expressed in C-fibers but to what extent are they expressed in A-delta fibers? There seems to be special cases where they are expressed in A-delta and dorsal horn neurons themselves, but is it generally safe to say that TRPV1 is predominantly in C-fibers? Also, any input as to which subclass of C-fibers have the most TRPV1 (e.g., peptidergin vs non-peptidergic) would be helpful.
TRPV1 is predominantly in C-fibers, and relatively more in peptidergic vs non-peptidergic C-fibers.
Thanks for your reply Zhen-Zhong. Do you know of any good references that summarize this?
This review can help
http://www.annualreviews.org/doi/full/10.1146/annurev.neuro.24.1.487?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
Thanks. To follow up on Vlad's link in case anyone else is interested, page 491-492 addresses the issue. I still need to cross-check the references cited, but it is stated that TRPV1 expression in C-fibers is considerably greater than in A delta fibers.
http://www.annualreviews.org/doi/full/10.1146/annurev.neuro.24.1.487?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
http://www.annualreviews.org/doi/full/10.1146/annurev.neuro.24.1.487?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
There are no in vivo studies showing definite evidence that identified A delta neurons do not have a physiologically significant expression of TRPV1. Bear in mind that some of the effects of capsaicin cannot be explained solely by its supposedly higher expression in peptidergic, trkA+ve C-fibre neurons.
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