6 Questions 8 Answers 0 Followers
Questions related from Steve J Sullivan
For fluorescent immunohistochemistry I fixed spinal cords in PFA, incubated in 30% sucrose, froze in OCT, and sectioned at 30um. All my sections are now on untreated charged glass slides that...
25 April 2023 4,414 4 View
Can anyone share their experience on the lifetime of different tracers or dyes? I am retrogradely labeling neurons with cholera toxin (CtB) 594 for chronic in-vivo imaging. I assume the lifetime...
29 October 2019 9,651 3 View
I'm 2-photon imaging stimulation-evoked calcium responses of deep neurons using a transgenic GCaMP6s mouse line. The neurons are quiet, meaning their baseline Fluorescence (F) is rather low. The...
28 February 2019 2,247 3 View
I am performing stereotaxic injection in mice. In the Paxinos Mouse Brain Atlas it appears that the depth is relative to the brain surface at bregma (z=0). The hole I drill in the skull is tiny,...
06 November 2018 2,860 4 View
Using immunohistochemisty, I want to determine if a postsynaptic protein is juxtaposed to C-fibers in lamina II of the spinal cord superficial dorsal horn. There are plenty of marker proteins for...
26 April 2016 9,190 1 View
I'm interested in immuno-labelling ganglion cells in mouse retina. I am mostly interested in antibodies that would also stain the dendrites (inner plexiform layer), but it's fine if it also labels...
22 October 2014 6,467 9 View