Steve J Sullivan

7 Questions 13 Answers 0 Followers

Questions related from Steve J Sullivan

How to help cryosectioned tissue adhere to glass slides for immunohistochemistry?

For fluorescent immunohistochemistry I fixed spinal cords in PFA, incubated in 30% sucrose, froze in OCT, and sectioned at 30um. All my sections are now on untreated charged glass slides that...

25 April 2023 4,430 4 View

How long do alexa dyes continue to fluoresce after loading neurons for long-term imaging?

Can anyone share their experience on the lifetime of different tracers or dyes? I am retrogradely labeling neurons with cholera toxin (CtB) 594 for chronic in-vivo imaging. I assume the lifetime...

29 October 2019 9,670 3 View

How to account for low background in quantifying calcium signals using deltaF/F?

I'm 2-photon imaging stimulation-evoked calcium responses of deep neurons using a transgenic GCaMP6s mouse line. The neurons are quiet, meaning their baseline Fluorescence (F) is rather low. The...

28 February 2019 2,256 3 View

What is the typical distance from the surface of the skull to the brain in adult mice?

I am performing stereotaxic injection in mice. In the Paxinos Mouse Brain Atlas it appears that the depth is relative to the brain surface at bregma (z=0). The hole I drill in the skull is tiny,...

06 November 2018 2,879 4 View

Does anyone know of dorsal horn proteins that are postsynaptic exclusively to C-fiber terminals?

Using immunohistochemisty, I want to determine if a postsynaptic protein is juxtaposed to C-fibers in lamina II of the spinal cord superficial dorsal horn. There are plenty of marker proteins for...

26 April 2016 9,204 1 View

What proportion of TRPV1 expressing DRG neurons are C-fibers vs A-delta fibers?

I'm recording capsaicin evoked mEPSCs in dorsal horn slices and I'm curious which pain terminals these originate from. I gather from the literature that TRPV1 receptors are mostly expressed in...

29 July 2015 5,316 7 View

Does anyone know of good retinal ganglion cell specific markers/antibodies that also label dendrites?

I'm interested in immuno-labelling ganglion cells in mouse retina. I am mostly interested in antibodies that would also stain the dendrites (inner plexiform layer), but it's fine if it also labels...

22 October 2014 6,477 9 View