What parameters of SDS band can we analyse using ImageJ software & How?

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SDS page software for analysis?

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Why are my bands weird in this Agarose gel?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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Are protein samples in SDS compatible with ELISA?

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Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

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Amanda Vazquez

If you have a protein marker, you can estimate your protein´s molecular weight. With ImageJ, you can also estimate purity of your samples.

Gisela Beutner

You can also determine the relative abundance of a protein by dividing the signal intensity of your protein over the signal of a marker protein.

Arunkumar Padmanaban

You can also work on western blotting post-SDS, if you are interested in a particular protein. Peptide mapping is also another analytical technique that can be performed post-SDS. Apart from these as earlier researchers suggest, Molecular weight determination, purity, integrity are the main parameters that is looked upon.