Which type of enrichment method is best for RNA sequencing (mRNA capture or Ribo depletion)?

11 October 2023 1 9K Report

I want to know about the best method for RNA sequencing- mRNA capture or Ribo depletion.

mRNA capture enriches protein-coding transcripts, enhances gene expression analysis, and quantifies mRNA levels. It reduces RNA population complexity, improving sensitivity and specificity. However, it may exclude non-coding RNA species for certain experiments.

Ribosomal RNA (rRNA) depletion is a comprehensive method for studying a wide range of RNA species, including mRNAs and non-coding RNAs. It offers advantages like capturing the entire transcriptome but may dilute sequencing depth for other RNA species.

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