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Questions related from Amanda Vazquez
I am making LPS monolayers to mimic peptide interaction with the outer membrane of Gram negative bacteria. I've read that is recommended to use rough LPS or rough/smooth mixtures of the endotoxin,...
12 December 2018 2,623 5 View
I’m determining esterase activity with pNPB as substrate, using Tris-HCl buffer. In optimal pH assays (ranging from 2-12), I’m observing substrate hydrolysis in pH 7 and higher; the intensity of...
16 February 2018 3,747 3 View
Hi, we are thinking about PEGylating a protein, for which dimerization or oligomerization seems to be important in its activity. Could this be affected by attaching PEG? Thanks.
16 January 2017 2,429 6 View
Hello. We are going to use the 5 Prime cell-free expression system in our lab, to obtain a recombinant peptide of around 5 kDa. In most references is mentioned that the expression is successful...
10 January 2017 5,294 4 View
Hello. I´m trying to grow yeasts (S. cerevisiae and C. albicans) in both YEPD and Sabouraud agar, but I don´t see any colonies even after 48 h of incubation at 28 or 37ºC. What could be happening?...
17 November 2016 8,300 9 View
The fusion protein I´m working with stains well with coomassie, but not with silver. After the fusion hydrolysis, the digestion subproducts are stained both with silver and coomassie, but I can´t...
16 September 2016 5,081 3 View
