Melting temperature of pure polyhydroxyvalerate ?

I am working on polyhydroxyalkanoates. While working I am getting meting temperature of the polymer is around 150 to 180 degree Celsius. From the structure its clearly visible the polymers are...

07 August 2018 5,427 5 View

Activated sludge media composition?

I want to produce PHA (Polyhydroxyalkanoates) from bacteria using activated sludge media but I want to know is there any composition available to make Activated sludge media so that I can prepare...

07 August 2016 8,310 2 View

Is there any protocol to get colored image of bacteria in Scanning electron microscope?

Actually I want to take surface image of Bacteria but generally I am doing it in black and whaite in Scanning electronmicroscope. But I saw image in cololed so what is the procedure or protocol to...

06 July 2016 664 2 View

Can anybody tell me the exact procedure or protocol for detection of PHA granule in side the bacteria?

I have see many articles explained for detection of PHA granule but I am not able to get it properly.How I will go for sectioning of PHA bacteria and by what chemical I will use to dehydrate it...

10 November 2014 5,433 1 View

Can anybody suggest a protocol for identification of proteins attached on the surface of poly-hydroxybutyrate granule in bacteria?

I want to extract and identify different types of protein in SDS that attached to the polyhydroxybutyrate granule which are intracellular protein, please suggest me any protocol that will be best...

10 November 2014 4,593 6 View

How does one get to know the position alpha/beta hydro-lase super family and c N terminus of a predicted enzyme?

I have predicted Polyhydroxyalkanoates synthase (PHAC) enzyme by modeler. Now I want to know how I will be able to find out the position of the alpha/beta hydro-lase super family and C N terminus...

09 October 2014 8,712 1 View

Do you have a protocol on how to produce and extract Biosurfactant by using bacsillus strain?

Actually I am extracting Polyhydroxy butyrate from bacillus by using MSM media but I am discarding the supernatant of that media because my concern is to the pellet only,so I am expecting the...

08 September 2014 8,715 11 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View