11 November 2014 1 6K Report

I have see many articles explained for detection of PHA granule but I am not able to get it properly.How I will go for sectioning of PHA bacteria and by what chemical I will use to dehydrate it  then whether I will use any stain or not.

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Melting temperature of pure polyhydroxyvalerate ?

I am working on polyhydroxyalkanoates. While working I am getting meting temperature of the polymer is around 150 to 180 degree Celsius. From the structure its clearly visible the polymers are...

07 August 2018 5,553 5 View

What minimum % of cell viability in Polyhydroxyalkanoates can be consider that polyhydroxyalkanoates are noncytotoxic in nature?

I did MTT assay to check whether my extracted PHA is non-cytotoxic or not.So can anybody tell me what is the minimum % of cell viability that can be consider the particular material is...

07 August 2016 7,100 1 View

Activated sludge media composition?

I want to produce PHA (Polyhydroxyalkanoates) from bacteria using activated sludge media but I want to know is there any composition available to make Activated sludge media so that I can prepare...

07 August 2016 8,411 2 View

Is there any protocol to get colored image of bacteria in Scanning electron microscope?

Actually I want to take surface image of Bacteria but generally I am doing it in black and whaite in Scanning electronmicroscope. But I saw image in cololed so what is the procedure or protocol to...

06 July 2016 769 2 View

Can anybody suggest a protocol for identification of proteins attached on the surface of poly-hydroxybutyrate granule in bacteria?

I want to extract and identify different types of protein in SDS that attached to the polyhydroxybutyrate granule which are intracellular protein, please suggest me any protocol that will be best...

10 November 2014 4,693 6 View

How does one get to know the position alpha/beta hydro-lase super family and c N terminus of a predicted enzyme?

I have predicted Polyhydroxyalkanoates synthase (PHAC) enzyme by modeler. Now I want to know how I will be able to find out the position of the alpha/beta hydro-lase super family and C N terminus...

09 October 2014 8,814 1 View

Do you have a protocol on how to produce and extract Biosurfactant by using bacsillus strain?

Actually I am extracting Polyhydroxy butyrate from bacillus by using MSM media but I am discarding the supernatant of that media because my concern is to the pellet only,so I am expecting the...

08 September 2014 8,813 11 View

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