Melting temperature of pure polyhydroxyvalerate ?

I am working on polyhydroxyalkanoates. While working I am getting meting temperature of the polymer is around 150 to 180 degree Celsius. From the structure its clearly visible the polymers are...

07 August 2018 5,427 5 View

What minimum % of cell viability in Polyhydroxyalkanoates can be consider that polyhydroxyalkanoates are noncytotoxic in nature?

I did MTT assay to check whether my extracted PHA is non-cytotoxic or not.So can anybody tell me what is the minimum % of cell viability that can be consider the particular material is...

07 August 2016 7,001 1 View

Activated sludge media composition?

I want to produce PHA (Polyhydroxyalkanoates) from bacteria using activated sludge media but I want to know is there any composition available to make Activated sludge media so that I can prepare...

07 August 2016 8,310 2 View

Is there any protocol to get colored image of bacteria in Scanning electron microscope?

Actually I want to take surface image of Bacteria but generally I am doing it in black and whaite in Scanning electronmicroscope. But I saw image in cololed so what is the procedure or protocol to...

06 July 2016 664 2 View

Can anybody suggest a protocol for identification of proteins attached on the surface of poly-hydroxybutyrate granule in bacteria?

I want to extract and identify different types of protein in SDS that attached to the polyhydroxybutyrate granule which are intracellular protein, please suggest me any protocol that will be best...

10 November 2014 4,593 6 View

How does one get to know the position alpha/beta hydro-lase super family and c N terminus of a predicted enzyme?

I have predicted Polyhydroxyalkanoates synthase (PHAC) enzyme by modeler. Now I want to know how I will be able to find out the position of the alpha/beta hydro-lase super family and C N terminus...

09 October 2014 8,712 1 View

Do you have a protocol on how to produce and extract Biosurfactant by using bacsillus strain?

Actually I am extracting Polyhydroxy butyrate from bacillus by using MSM media but I am discarding the supernatant of that media because my concern is to the pellet only,so I am expecting the...

08 September 2014 8,715 11 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View