I was wondering if anyone might have experience with degradation of BV tandem dyes. We’ve been having a strange population appear in our experiments every once in a while and were able to trace it to a tandem dye degrading and emitting on the channel of the donor molecule. But we still can’t figure out what might be causing it. Sometimes it would be only one replicate out of three, which is really confusing. It seems to happen to certain people more than others so might be something about sample handling technique. Maybe someone had a similar thing happen?