Greetings,
I am wondering which micropollutant I may be able to see with HPLC analysis (no tandem MS), for example diclofenac or ibuprofen. I would test on spiked water samples, so theoretically I wouldn't need an extraction phase. Which column are indicated? Which mobile phases ?
Thanks.
Hello Maurizio Cuomo , you can measure a lot of micropolltants with HPLC. My researches deals intensively with pharmaceuticals. You are welcome to have a look at my paper, then you will see which eluents, columns etc. I normally used.
If you want to work with spiked samples, of course it depends on which detection-system you use. It is generally recommended to measure the water samples unspiked, almost as a blind sample, so that you can see exactly what differences are between spiked and unspiked samples.
Hello Maurizio Cuomo, if you are going to deal with pesticides, you can have a look at my paper.
Article Imidacloprid elimination by O3 and O3/UV: kinetics study, ma...
In general, C18 column is widely used. One of the important things is the dimension of the column. Not only because of length should fit the HPLC but also particle size of the column may affect your results. Furthermore, max pressure and peak detention time are also affected by the dimensions of the column.
Best,
Busra S. Baghirzade
Using HPLC Method with DAD Detection for the Simultaneous Determination of 15 Drugs in Surface Water and Wastewater
Irena Baranowska, Bartosz Kowalski
Pol. J. Environ. Stud. 2011;20(1):21–28
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