How to solve spikes and peaks in interpolated raster surfaces with Kriging?

25 November 2020 9,657 4 View

Anyone used Presenilin 1 [C1C2] Antibody by GeneTex?

Hello, I'm performing WB analysis using the Antibody anti-Presenilin 1 [C1C2] (cat number GTX116016) of the GeneTex company. The datasheet specifies that this antibody recognizes the full-length...

03 August 2020 7,086 1 View

Long-term forest data?

Does anybody know if some datasets on trees health or growth time series are freely available for Europe? My goal would be to study the climate change between 1901 and 2019 and to compare it with...

30 July 2020 3,201 4 View

What micropollutants can I analyze on HPLC ?

Greetings, I am wondering which micropollutant I may be able to see with HPLC analysis (no tandem MS), for example diclofenac or ibuprofen. I would test on spiked water samples, so theoretically I...

03 June 2020 3,723 3 View

How to treat mammalian diel activity patterns as a continuous variable?

Does anyone have experience in treating/converting mammalian diel activity patterns (diurnal/nocturnal/crepuscular/cathemeral) as a continuous variable rather than a factorial one? I am thinking...

13 May 2020 9,562 2 View

How do you make your analysis scripts 'reviewable' when there is a lot of bulky code repititions?

Hey everyone! How do you make your code readable for other people when there are long chunks of code which you repeat a lot (e.g. the same fancy figure for 10 DVs)? My current solution: I...

12 February 2020 549 1 View

Parallel computing and graphic CPU (GCPU) usage in R, is it possible with ALL R packages?

Hi all, surfing the web I wasn't able to find a simple and effective answer to my question. In other words, I need to speed up a code I wrote using several packages such as raster, rgdal, biomod2...

13 December 2019 1,703 4 View

HPLC analysis - why do I have different area values for the same sample?

Good morning, I am currently doing some standards for my HPLC. My interest compound is butyric acid and I am injecting it in four different concentrations in order to build the standard and the...

15 August 2019 9,049 5 View

Any suggestion for a low volume reverse osmosis filtration system?

Dear all, I am looking for a reverse osmosis system to concentrate a sugar solution at laboratory scale. The volumes I am going to treat are quite small, 10 - 50 mL and therefore I am looking for...

10 June 2019 4,280 2 View

HPLC analysis for VFA - how to correctly do an HPLC run?

Hello everyone, I've been using an HPLC for VFA detection but I have little experience. I'm specifically looking for acetate, propionate, butyrrate, valerate, etc. . I'm working with a c18 column...

28 May 2019 4,152 7 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

A three electrode sensor inkjet printed gets damaged in few hours of usage in a dynamic media flow chip, any solution for that?

We are using some biosensors which are inkjet printed and are used in the microfluidic chip where media is flowing at the flow rate of around 60 uL/min. However, after the continuous use of few...

05 January 2021 610 2 View

How to clean LEDs (InGaN on SiC) and Ti/Au electrode chips?

I need perfect cleanliness for further processes but I am not sure which cleaning method would be sufficient yet letting the layers intact.

01 December 2020 1,099 2 View

Why my IP injcetion drug for mice turn opaque after thawing?

Hi, I have prepared a drug solution that is dissolved in DMSO. We are giving IP injection to mice. The final stock concentration is 2mg/ml in 5% DMSO, 2% Tween-80 and 93% Saline. Before injection,...

18 November 2020 6,000 2 View

What protein about 100kDa is tyrosine phospholated when challenge T cell with anti CD3?

Hi, I uesd purified mouse CD3 T cell from spleen and stimulated these cells with anti mouse CD3 for different time. At least I can say two proteins are activated (black and red arrow). For the...

11 November 2020 7,202 3 View

The algal cell always gather together when the cells concentration is high and those cells would block the microfluidic chips. How can i handle it?

I want to pump a high concentration algal cells into microfluidic chip, however those cells are always gather together forming a clusters to block the connection between Teflon tube and...

26 October 2020 5,366 2 View

Can any one tell me that how we represent RFID chip (Z=23-j224) with a discrete port in CST?

Dear All, I am facing trouble in making antenna for RFID tag. Just tell me that how a chip resistance represents by discrite port in CST? for example I am using NXP chip with...

22 October 2020 7,968 2 View

Have spin-based devices found any appilcation in medical science?

Spin-based devices such as magnonic chips, spin-torque oscillators mostly find their application in signal processing, computing, etc. Is there any research work focusing on developing magnonic...

17 October 2020 3,459 1 View

Problem about rat anesthesia?

I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the...

07 October 2020 9,140 7 View