I am currently dealing with the purification of filamentous cyanobacteria from a mixed culture of cyanos and green algae (Scenedesmus, Desmodesmus, Chlorella etc.).
In our lab equipment and chemicals are limited so that we can largely only count with mechanical removal and removal via chemical treatment.
My thoughts are to combine differential settling, microfiltration with vacuum filtration FILTERMAX with 0.22 micrometer, and treatment with 0.5 mM CuSo4, in this order.
However, I am not finding any paper or protocol online that deals with this kind of problem.
Could anyone please advice if these techniques are suitable, provide some literature and ideally suggest a way how to assess the effectiveness of the removal apart from microscopy and cell counting?
Thank you a lot in advance1
Hello,
I recommend these papers:
- Isolation and Culturing Axenic Microalgae: Mini–Review
Saúl Fernandez-Valenzuela et al.,2021
-Axenic cultures for microalgal biotechnology: Establishment, assessment, maintenance, and applications.
Chau Hai Thai Vu et al., 2017
-A method of batch-purifying microalgae with multiple antibiotics at extremely high concentrations.
HAN Jichang et al.; 2016
Good luck
To control green microalgae contamination in Blue-green algae cultures adding Dichlorvos chemical could be a chance.
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